Author name: Upshan

Measurement of bacterial growth using UV spectrophotometer

Measurement of bacterial growth using UV spectrophotometer

Biology /

Understanding bacterial growth is crucial in various fields, from medicine to biotechnology. One commonly used method for quantifying bacterial growth is through the use of a UV spectrophotometer. This technique relies on the principle that bacteria, like many other biological molecules, absorb light at specific wavelengths. What is Spectrophotometer? An equipment used in science to […]

Measurement of bacterial growth using UV spectrophotometer Read More »

Dental caries susceptibility tests Snyder test

Dental caries susceptibility tests: Snyder test

Biology /

The Snyder test, a simple and inexpensive microbiological assay, provides a measure of an individual’s susceptibility to dental caries (tooth decay). It relies on the principle that the presence of specific bacteria in the oral cavity, particularly lactobacilli, is a strong indicator of the potential for caries development. These bacteria ferment sugars, producing acids that erode

Dental caries susceptibility tests: Snyder test Read More »

Types of Forest in Nepal

Types of Forest in Nepal

Biology /

Nestled in the heart of the Himalayas, Nepal is a land of breathtaking landscapes, towering peaks, and a rich tapestry of diverse forests. These verdant havens, spread across various altitudinal zones, are not just a breathtaking sight but a vital resource for the country’s ecology, biodiversity, and human communities. Let’s delve into the fascinating world

Types of Forest in Nepal Read More »

Phenylalanine deaminase test Principle, Requirements, Procedure and Results interpretations

Phenylalanine deaminase test: Principle, Requirements, Procedure and Results interpretations

Biology /

The phenylalanine deaminase test is a biochemical test used to identify bacteria that possess the enzyme phenylalanine deaminase. This enzyme plays a crucial role in bacterial metabolism, allowing them to break down the amino acid phenylalanine into phenylpyruvic acid and ammonia. Principle of the Phenylalanine deaminase test The PDA test leverages the specific enzymatic activity

Phenylalanine deaminase test: Principle, Requirements, Procedure and Results interpretations Read More »

Salmonella-Shigella Agar test Principle, Composition, Procedure, Results interpretation

Salmonella-Shigella Agar test: Principle, Composition, Procedure, Results interpretation

Biology /

The Salmonella-Shigella (SS) agar is a selective and differential culture medium specifically designed for the isolation and identification of Salmonella and Shigella species from clinical specimens. These bacteria are notorious for causing foodborne illnesses, commonly known as salmonellosis and shigellosis. The SS agar employs a unique combination of ingredients that suppress the growth of unwanted

Salmonella-Shigella Agar test: Principle, Composition, Procedure, Results interpretation Read More »

DNA Microarray Principle, Types and steps involved in cDNA microarrays

DNA Microarray: Principle, Types and steps involved in cDNA microarrays

Biology /

What do you mean by DNA Microarray? In molecular biology, DNA microarrays, sometimes referred to as gene chips, are potent instruments that enable scientists to test thousands of genes’ expression levels at once. In essence, they are tiny labs on a chip that can produce enormous volumes of information regarding the activity of genes in

DNA Microarray: Principle, Types and steps involved in cDNA microarrays Read More »

MRS Broth test Principle, Composition, Procedure, and Results interpretation

MRS Broth test: Principle, Composition, Procedure, and Results interpretation

Biology /

What do you mean by MRS Broth test? The MRS Broth test is a crucial tool in the world of microbiology, specifically when working with a specific group of bacteria called Lactobacillus. This test helps us understand if a particular sample contains these important bacteria and, if so, how effectively they can ferment specific sugars. Let’s

MRS Broth test: Principle, Composition, Procedure, and Results interpretation Read More »

Bacitracin test Principle, Requirements, Procedure, Results interpretations, Limitations

Bacitracin test: Principle, Requirements, Procedure, Results interpretations, Limitations

Biology /

What do you mean by Bacitracin test? A microbiological test called the Bacitracin test is used to identify specific types of bacteria, namely group A streptococci, which are also referred to as Streptococcus pyogenes. What do you mean by Bacitracin ? Although use in animals leads to antibiotic resistance, bacitracin is a polypeptide antibiotic used

Bacitracin test: Principle, Requirements, Procedure, Results interpretations, Limitations Read More »