| Course Title : | Methods in Microbiology |
| Course No : | MB 405 (Applied Microbiology) |
| Nature of the Course : | Theory |
| Full Marks : | 100 |
| Pass Marks : | 35 |
| Year : | Fourth ( 4th) |
| Total Lecture Hours : | 150 |
Course Objectives
After completion of this course Methods in Microbiology , the students will be able to:
- understand the principles, procedures and applications of methods used in the fields of
microbiology
Course Contents of Methods in Microbiology
The Course contents of Methods in Microbiology ,
| S.N | Topic / Chapter | Lecture Hours |
| 1 | Safety Measures in Microbiology Laboratory Principles of laboratories safety, biosafety level of laboratories and bio-hazards, risk group of microorganisms, decontamination and safe disposal of contaminated materials, sterilization and sterility techniques | 10 hrs |
| 2 | Methods of Specimen Collection, Transportation and Processing of Clinical Samples for Bacteria Detection Cerebrospinal fluid, blood and other body fluids, sputum, urine, discharges and pus, stool, culture procedures, test algorithms for diagnosis of bacteria, antibiotic susceptibility tests (Kirby Bauer disc diffusion method, minimum inhibitory concentration determination) | 20 hrs |
| 3 | Method of Collection, Transportation and Processing of Clinical Samples for Virus Detection Introduction, types of samples, maintenance of temperature and transportation, identification and interpretation, culture of virus in chick embryo and cell lines, cytopathic effects, detection of virus from culture, serological tests for the diagnosis of viruses | 20 hrs |
| 4 | Sample Collection and Laboratory Diagnosis of Mycotic Infections Samples for fungal infections, sputum, nasal swab, skin scraping, hair and nails, CSF for fungal meningitis, microscopy, staining, culture | 10 hrs |
| 5 | Method of Collections of Samples and Processing for Detection of Parasites Introduction, types of samples for parasite detection, sample processing and detection methods for blood, stool and tissue parasites | 15 hrs |
| 6 | Immunological and Serological Tests Principles, procedures, advantages and applications of precipitation, agglutination, complement fixation, ELISA, radio-immunoassay | 15 hrs |
| 7 | Method of Collections of water Samples and Microbiological Analysis Introduction, types of water samples, water sample processing and detection methods, MPN, MF, BOD | 10 hrs |
| 8 | Field Level Tests for Disease Diagnosis Principles, procedures and applications of rapid tests for malaria, kala-azar, lymphatic filariasis, dengue, HIV, HBV, HCV, rotavirus, JE, leptospirosis, typhoid | 10 hrs |
| 9 | Molecular Tests in Microbiology Laboratories Samples for molecular diagnostic tests, DNA/RNA extraction, PCR, Real Time PCR, PCR-RFLP, sequencing, western blotting | 15 hrs |
| 10 | Microbiological Quality Tests of Foods Quality test of milk and milk products, egg and egg products, meat and meat products, cereal and cereal products, HACCP, detection methods of carcinogens and toxins in food | 15 hrs |
| 11 | Microbiology Laboratory in Agriculture Methods for preparation of bio-fertilizers, detection methods of pesticide, herbicide, insecticide, fungicide in soil, isolation and detection of pectinolytic, lignolytic, lipolytic, cellulolytic microorganisms from soil | 10 hrs |
| Total Lecture Hours | 150 |
Recommended Readings
For Methods in Microbiology we have,
Text books
The text books for Methods in Microbiology are listed ,
- Cheesbrough M (2007). Medical Laboratory Manual for Tropical Countries Vol. 2 ELBS London.
- Brown AE (2012). Benson’s Microbiological Applications. Laboratory Manual in General
Microbiology. (12th edition). McGraw-Hill Publisher. - Collee JG, Fraser AG, Marmion BP and Simmons A (1996). Mackie &McCartney Practical Medical
Microbiology (14th edition). Churchill Livingstone.