| Course Title : | Bioinstrumentation |
| Course No : | MB 407 (Interdisciplinary subject) |
| Nature of the Course : | Theory |
| Full Marks : | 50 |
| Pass Marks : | 17.5 |
| Year : | Fourth( 4 th) |
| Total Lecture Hours : | 75 |
Course Objectives
After completion of this course Bioinstrumentation , the students will be able to:
- understand the working principles and procedures of instruments used in microbiology laboratory
Course Contents
| S.N | Topic / chapter | Lecture Hours |
| 1 | Buffers Physiological solutions and buffers, importance of buffers in biological researches | 2hrs |
| 2 | General Principle and Approaches of Biochemical Investigations Introduction, concept, significance of disruption of cells, extraction buffer, chemical methods, enzymatic digestion, mechanical methods, selection of protein source, methods of solubilization of protein, solubility of protein, chromatographic separations, electrophoresis, ultracentrifugation of protein samples | 8hrs |
| 3 | Working principle, instrumentation and application of Phase contrast, electron microscopy, fluorescence microscopy | 10hrs |
| 4 | Working Principle, Instrumentation and Applications of Centrifugation techniques, Electrophoretic techniques: Agarose gel, Polyacrylamide gel electrophoresis, Isoelectric focusing, Two- dimensional electrophoresis | 15hrs |
| 5 | Principles, Instrumentation and Uses of Chromatographic Techniques Ion exchange chromatography, Affinity Chromatography, Paper and Thin layer chromatography, Gel Permeation chromatography, Gas Chromatography, High performance Liquid Chromatography (HPLC) | 20 hrs |
| 6 | Principle, Instrumentation and Application of Lambert Beer’s law, X-ray and gamma ray spectroscopy, spectrofluorometry, optical rotation dispersion, electron spin resonance (ESR), Infrared spectroscopy, nuclear magnetic resonance spectroscopy, mass spectrometry, ultraviolet and visible spectroscopy | 10hrs |
| 7 | Principle, Instrumentation and Application of Molecular Techniques Thermocycler, Probes, Sequencer, Gel documentation system, Microarray | 10hrs |
| Total Lecture Hours | 75hrs |
Recommended Readings
Text Books for Bioinstrumentation
- Skoog DA, Holler FJ and Nieman TA (2005), Principles of Instrumental Analysis, 5th Edition, Thomson
Books/Cole - Wilson K and Walker J (Eds) (2005), Principles and Techniques of Biochemistry and Molecular
Biology,6thEdition, Cambridge University Press - Mendham J, Denny RC, Barnes JD and Thomas M (2008), Vogel’s Text Book of Quantitative Chemical
Analysis, 6th Edition, Pearson Education .