Author name: Sudan Pokhrel

Strategies for biodiversity conservation

Strategies for biodiversity conservation

Biology /

Biodiversity conservation necessitates a multidimensional approach that includes both in-situ and ex-situ initiatives, strong laws and regulations, community engagement, scientific research, and sustainable behaviors. By implementing these techniques, we can maintain and preserve Earth’s biodiversity, preserving ecosystem resilience and future generations’ well-being. Biodiversity conservation entails safeguarding, preserving, and managing ecosystems, species, and genetic variety to […]

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Fluorescent in-situ hybridization advantages, limitations and preparation of probe

Fluorescent in-situ hybridization: advantages, limitations and preparation of probe

Biology /

Fluorescent in-situ hybridization (FISH) is a very effective and adaptable technology for detecting and localizing specific nucleic acid sequences in cells and tissues. Its advantages include high sensitivity and specificity, spatial resolution, and fast results, making it ideal for clinical diagnostics and genetic research. However, FISH has limitations, including resolution restrictions and the complexity of

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Fluorescent in-situ Hybridization to Diploid Cells

Fluorescent in-situ Hybridization to Diploid Cells

Biology /

Fluorescent in-situ hybridization (FISH) is a versatile and effective method for identifying and localizing particular nucleic acid sequences in diploid cells. FISH is extremely useful in clinical diagnostics, genetic research, and cell biology because it provides precise spatial and temporal information on gene expression, chromosomal abnormalities, and genomic organization. To use FISH successfully, each step

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Immunostaining of developing Embryos of Drosophila

Immunostaining of developing Embryos of Drosophila

Biology /

Immunostaining of developing Drosophila embryos is an important approach in developmental biology because it allows researchers to see the location and expression of certain proteins. Immunostaining is important for studying genetics, cell biology, and embryogenesis in Drosophila and other model organisms because it provides precise insights into protein function and developmental processes. Immunostaining Immunostaining is

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Germplasm conservation- An Overview

Biology /

Germplasm conservation is vital to guaranteeing food security, protecting biodiversity, and promoting sustainable agriculture. The conservation of genetic resources serves as the foundation for breeding programs, research, and the production of robust and productive crops and animals. Despite tremendous progress made through worldwide initiatives and enhanced conservation approaches, obstacles like as genetic loss, budgetary limits,

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Cryopreservation Principle, Process, limitations and precautions

Cryopreservation: Principle, Process, limitations and precautions

Biology /

Cryopreservation is a powerful technique that plays a critical role in various scientific and medical fields. Many opportunities for study, medicine, agriculture, and conservation are made possible by its capacity to stop biological processes and preserve cells, tissues, and organs for lengthy periods of time. On the other hand, knowing and perfecting the underlying concepts,

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Protocol for protoplast isolation

Protocol for protoplast isolation

Biology /

Protoplast isolation is a delicate process that requires careful attention to detail and sterile techniques. Protoplast Isolation, researchers can obtain viable and high-yield protoplasts suitable for various applications in plant biotechnology, from genetic transformation to cellular and molecular studies. Protoplast Isolation Protoplast isolation is a technique used in plant biology to remove the cell wall

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