Author name: Sudan Pokhrel

Satellitism test for identification of Haemophilus influenzae

Satellitism test for identification of Haemophilus influenzae

Haemophilus influenzae requires both X factor (hemin) and V factor (NAD) for growth, which are not readily available in all growth media but can be produced by certain other bacteria. Haemophilus influenzae Haemophilus influenzae is a small, pleomorphic, Gram-negative coccobacillus that is a significant human pathogen. It is part of the normal flora of the […]

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4-Methylumbelliferyl-β-D-Glucuronide (MUG) Test

4-Methylumbelliferyl-β-D-Glucuronide (MUG) Test

4-Methylumbelliferyl-β-D-Glucuronide (MUG) test is a biochemical assay used to detect the enzyme β-glucuronidase, which is produced by certain bacteria, notably Escherichia coli (E. coli). This test is commonly used in microbiological water testing and food safety to identify E. coli as an indicator of fecal contamination. 4-Methylumbelliferyl-β-D-Glucuronide Test The 4-Methylumbelliferyl-β-D-Glucuronide test (MUG) is a biochemical

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Optochin susceptibility test Principle, Procedure and Results interpretation

Optochin susceptibility test: Principle, Procedure and Results interpretation

Optochin susceptibility test is a key diagnostic tool used to differentiate Streptococcus pneumoniae from other alpha-hemolytic streptococci, particularly Streptococcus viridans group. The test is based on the sensitivity of S. pneumoniae to the antibiotic optochin, which inhibits its growth. Optochin Susceptibility Test Optochin Susceptibility Test is a laboratory method used to differentiate Streptococcus pneumoniae from

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Hippurate hydrolysis test Principle, Procedure, Result interpretations and Limitations

Hippurate hydrolysis test: Principle, Procedure, Result interpretations and Limitations

Hippurate hydrolysis test is a biochemical experiment used to distinguish between specific bacteria species, particularly those from the Streptococcus and Campylobacter genera. This test is mostly used to identify Group B Streptococcus (Streptococcus agalactiae) and Campylobacter jejuni. Hippurate Hydrolysis Test The hippurate hydrolysis test is a biochemical experiment that distinguishes and identifies certain bacterial species

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Bile solubility test Principle, Procedure, and Result

Bile solubility test: Principle, Procedure, and Result

Bile solubility test is used to differentiate Streptococcus pneumoniae from other alpha-hemolytic streptococci based on the ability of S. pneumoniae to lyse in the presence of bile salts. Bile solubility test Bile solubility test is based on the unique ability of Streptococcus pneumoniae to lyse in the presence of bile salts (typically sodium deoxycholate). This

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Phytoremediation Classification, Mechanisms, Applications and limitations

Phytoremediation: Classification, Mechanisms, Applications and limitations

Phytoremediation represents a viable and environmentally acceptable approach for environmental cleanup. Phytoremediation promotes long-term environmental management and restoration by utilizing plants’ inherent ability to absorb, convert, and detoxify contaminants. Understanding its classification, mechanisms, applications, and limitations is critical for successfully implementing phytoremediation procedures and addressing environmental concerns. Phytoremediation Phytoremediation is an environmentally benign and cost-effective

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Biofertilizer- Advantages, Types, methods of application and Disadvantages

Biofertilizer- Advantages, Types, methods of application and Disadvantages

Biofertilizers are essential to sustainable agriculture because they use natural processes to improve plant growth and soil fertility. They are a great substitute for chemical fertilizers due to their advantages in the areas of economy, agronomy, and the environment. However, knowledge of this kinds, application techniques, and potential drawbacks is essential for successful use. Biofertilizer

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Acetate utilization test Principle, media composition, Procedure and Results

Acetate utilization test: Principle, media composition, Procedure and Results

Acetate utilization test measures an organism’s capacity to use acetate as its only source of carbon. This is especially important for distinguishing members of the Enterobacteriaceae family. Organisms that can grow on acetate create an alkaline reaction, which changes the medium’s pH indicator from green to blue. Acetate utilization test Acetate utilization test is a

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