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Cryopreservation Principle, Process, limitations and precautions

Cryopreservation: Principle, Process, limitations and precautions

Biology /

Cryopreservation is a powerful technique that plays a critical role in various scientific and medical fields. Many opportunities for study, medicine, agriculture, and conservation are made possible by its capacity to stop biological processes and preserve cells, tissues, and organs for lengthy periods of time. On the other hand, knowing and perfecting the underlying concepts, […]

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Protocol for protoplast isolation

Protocol for protoplast isolation

Biology /

Protoplast isolation is a delicate process that requires careful attention to detail and sterile techniques. Protoplast Isolation, researchers can obtain viable and high-yield protoplasts suitable for various applications in plant biotechnology, from genetic transformation to cellular and molecular studies. Protoplast Isolation Protoplast isolation is a technique used in plant biology to remove the cell wall

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Novobiocin susceptibility test Principle, Procedure and Results interpretations

Novobiocin susceptibility test: Principle, Procedure and Results interpretations

Biology /

Novobiocin susceptibility test is primarily used to distinguish between species of coagulase-negative staphylococci, specifically Staphylococcus saprophyticus, which is novobiocin-resistant, from other coagulase-negative staphylococci, such as Staphylococcus epidermidis, which is novobiocin-sensitive. Novobiocin susceptibility test Novobiocin susceptibility test is a diagnostic process used in microbiology laboratories to evaluate if a bacterium is susceptible (sensitive) or resistant to

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Somaclonal variation Basis, Applications and limitations

Somaclonal variation: Basis, Applications and limitations

Biology /

Somaclonal variation, a fascinating phenomenon in plant tissue culture, refers to the genetic and phenotypic variations observed in plants regenerated from cultured cells or tissues. This inherent ability of plants to exhibit variability after a period in vitro (outside the living organism) presents a powerful tool for plant breeders and researchers. This comprehensive overview delves

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L-Pyrrolidonyl Arylamidase (PYR) test Principle, Requirements, Procedure and Results

L-Pyrrolidonyl Arylamidase (PYR) test: Principle, Requirements, Procedure and Results

Biology /

L-Pyrrolidonyl Arylamidase (PYR) test is a biochemical assay used in microbiology to detect specific bacterial species, specifically streptococci vs enterococci. The test detects the enzyme L-pyrrolidonyl arylamidase. L-Pyrrolidonyl Arylamidase (PYR) L-Pyrrolidonyl Arylamidase (PYR) is a bacterial enzyme that breaks down the substrate L-pyrrolidonyl-β-naphthylamide. The PYR test, a quick and specific biochemical assay used in microbiology

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Hematopoiesis Types of Haematopoietic stem cells, Process and Regulation

Hematopoiesis: Types of Haematopoietic stem cells, Process and Regulation

Biology /

Hematopoiesis is the process by which all blood cells are produced from hematopoietic stem cells (HSCs) in the bone marrow. This process is vital for maintaining the proper function and balance of the blood and immune systems. Hematopoiesis The process by which all blood cells are created from hematopoietic stem cells (HSCs) in the bone

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Amphotericin B mechanism, spectrum, pharmacokinetics, uses and side effects

Amphotericin B: mechanism, spectrum, pharmacokinetics, uses and side effects

Biology /

Amphotericin B is a broad-spectrum antifungal medication used to treat serious fungal infections. Due to its effectiveness against a wide range of fungi and its fungicidal (killing fungi) properties in certain situations, it remains a cornerstone therapy despite its potential for severe side effects Amphotericin B Amphotericin B is a broad-spectrum antifungal drug. It is

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Tinea capitis etiology, clinical manifestation, diagnosis and treatment

Tinea capitis: etiology, clinical manifestation, diagnosis and treatment

Biology /

Tinea capitis, often known as scalp ringworm, is a contagious fungal infection that affects the scalp, hair shafts, and occasionally the brows and eyelashes. It is caused by dermatophyte fungi, specifically Trichophyton and Microsporum species. These fungus feed on keratin, a protein found in hair, skin, and nails. Tinea capitis Tinea capitis, often known as

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