Butzler campylobacter medium composition, preparation and uses

Butzler campylobacter medium: composition, preparation and uses

Butzler Campylobacter

The selective culture medium known as Butzler Campylobacter medium is mostly used to isolate specific strains of Campylobacter, including Campylobacter jejuni and Campylobacter coli, which are frequently responsible for foodborne infections in people. Professor Jean-Paul Butzler, a Belgian microbiologist who made important contributions to the study of Campylobacter, is honored by the medium’s name. The medium facilitates the identification of these bacteria from clinical samples, including excrement, as well as potentially contaminated food and water sources.

Composition of Butzler Campylobacter Medium

Butzler Campylobacter medium’s constituents are specifically chosen to encourage Campylobacter development while stifling those of other species. This is an explanation of the typical composition:

Peptone: ten grams

Nitrogen, vitamins, and other vital nutrients that support bacterial growth can be found in peptone. It is produced by the enzymatic breakdown of proteins and supplies the building blocks required for the growth of Campylobacter.

Five grams of yeast extract

Additional vitamins and growth factors that improve bacterial metabolism are provided by yeast extract. It has a lot of B vitamins, especially those certain species of Campylobacter require.

Five grams of sodium chloride (NaCl)

Sodium chloride aids in preserving the medium’s osmotic balance, preventing excessive swelling or dehydration of the bacterial cells.

Agar: 12–15 grams

Seaweed is the source of agar, a solidifying agent. It gives the medium a gel-like consistency, giving bacterial colonies a stable surface on which to develop.

Antibiotics

Vancomycin dosage: 10 mg
Gram-positive bacteria, which are uninteresting in this medium, are inhibited in growth by this antibiotic.

2500 units of polymyxin B
Other than Campylobacter species, gram-negative bacteria are specifically inhibited by polymyxin B.
Trimethoprim dosage: 5 mg
While other bacteria cannot grow in this environment, Campylobacter can because trimethoprim inhibits the synthesis of folic acid.
Two milligrams of amphotericin B
This antifungal drug stops mold and yeast from growing, which could contaminate the culture.

Blood Optional: 5-7% (sheep or horse blood that has been defibrinated)

Blood is added to certain formulations to boost Campylobacter species’ recovery and supply additional nutrients. By giving the colonies more distinction, it also helps with visualizing.

One litre of distilled water

All the elements are dissolved by the action of water, which serves as a solvent and promotes optimal medium formation.

pH: Set at 7.4 ± 0.2.

The medium’s pH is adjusted to a neutral level, which is ideal for Campylobacter species growth.

Preparation of Butzler Campylobacter Medium

Butzler Campylobacter medium preparation entails the following steps:

Combining and Weighing

Weigh each component precisely in accordance with the amounts given. In distilled water, combine peptone,yeast extract, sodium chloride, and agar.

Mixing the Components:

Gently heat the mixture, stirring to dissolve all of the solid ingredients. Since the agar serves as a solidifying agent, make sure it is completely dissolved.

sterilisation

After the ingredients have been dissolved, autoclave the medium for 15 minutes at 121°C to sanitize it. To get rid of any unwanted bacteria that might be in the substances, this step is crucial.

cooling

After autoclaving, let the medium cool to a temperature of 45 to 50°C. The antibiotics that will be added in the following phase are protected from being destroyed by heat by cooling.

Including Blood and Antibiotics

Aseptically include the antibiotics (Amphotericin B, Trimethoprim, Polymyxin B, and Vancomycin) into the mixture when the medium has cooled. If blood is needed for the medium, add it now. In order to prevent the introduction of air bubbles or contamination, make sure to mix fully yet gently.

Pouring Dishes

Even distribution of the chilled medium should be ensured while pouring it into sterile Petri dishes in a sterile setting. Give the medium time to set.

Keep in mind

If not using the prepared plates right away, they should be kept in a refrigerator between 2 and 8°C. For quality, proper labeling with the preparation date is crucial.

Uses of Butzler Campylobacter Medium

In clinical, food, and water testing labs, Butzler Campylobacter medium is crucial. Isolating Campylobacter species, which are a major global source of gastrointestinal illnesses, is its main duty. Here are a few of the main applications for this medium:

Diagnosis Clincial

The most frequent causes of bacterial gastroenteritis in humans are Campylobacter jejuni and Campylobacter coli, which are frequently spread by tainted food and water. These bacteria are isolated from stool samples of infected persons using Butzler Campylobacter medium. Because the medium is selective, it makes it easier to recognize the bacteria, which speeds up the infection’s diagnosis and treatment.

Testing of food

Dairy products, unpasteurized milk, and poultry are frequently contaminated with Campylobacter. In food testing labs, Butzler Campylobacter medium is frequently used to check for the presence of these bacteria in food products. This lowers the possibility of foodborne outbreaks and ensures food safety.

Monitoring of Water Quality

Fecal matter-contaminated water has the ability to contain Campylobacter, which could cause outbreaks in nearby areas. In order to identify the presence of Campylobacter species in water, particularly in regions where waterborne illnesses are prevalent, Butzler Campylobacter medium is utilized in water testing.

Investigation

This media is used by researchers to cultivate Campylobacter in lab settings. Isolating the bacteria in selective media such as Butzler Campylobacter medium is a common first step in research pertaining to bacterial pathophysiology, antibiotic resistance, and novel therapeutic approaches.

Testing in the Environment

Environmental surfaces in food production facilities, hospitals, and public areas may be examined for Campylobacter contamination in addition to food and water testing. In order to stop the spread of bacteria, this helps guarantee that surfaces are thoroughly sterilized.

Advantages of Butzler Campylobacter Medium

Particular Character

Butzler Campylobacter medium is highly selective, allowing only Campylobacter species to thrive while blocking a wide range of other microbes. This is due to the addition of particular antibiotics.

Effective over a Range of Samples

Because of the medium’s versatility, Campylobacter can be isolated from a wide range of materials, such as water, food products, and clinical specimens.

Encourages the Formation of Pigment

Blood can make Campylobacter colonies stand out more in the media, making them simpler to identify as grey or translucent colonies.

In summary

The Butzler Campylobacter medium is a crucial instrument for the isolation and identification of Campylobacter species, which are accountable for a significant number of gastroenteritis cases across the globe. Its capacity to suppress rival bacteria and its selectivity make it perfect for use in research, food and water testing, and clinical diagnostics. By employing this medium, labs can guarantee precise Campylobacter identification and aid in the containment of diseases brought on by these microorganisms.

Frequently Asked Questions(FAQ)

Write about Gastrointestinal diseases?

The term “gastrointestinal diseases” (abbreviated “GI diseases” or “GI illnesses”) describes conditions that affect the esophagus, stomach, small intestine, large intestine, and rectum as well as the liver, gallbladder, and pancreas, which are the digestive system’s auxiliary organs.

What are the uses of Butzler Campylobacter?

In particular, Campylobacter bacteria are isolated and identified using Butzler Campylobacter medium from clinical samples, food samples, water quality monitoring, and bacterial infection studies.

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